FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Dannettiatripetala, 2,2-diphenyl-picrlhydrazyl (DPPH), scavenging activities, lipid profile
Dennettiatripetala belongs to the family Annonaceae. The aqueous and n-Hexane extract were screened for the presence of phenols, flavonoids and vitamin C and the 2,2-diphenyl-picrlhydrazyl (DPPH) radical scavenging activities of the extracts were also determined. Twenty five Wistar rats of either sex, weighing 130-140g, were used for this study. The animals were grouped into five Groups: A, B, C, D and E of five animals each. The control group animals were served single oral daily doses of normal saline, while the experimental groups B, C, D and E received 1.0, 1.5, 2.0 and 2.5 mg/kg b.w of the aqueous extract for 10 days, respectively. Twenty four hours after the last administration, the animals were sacrificed under chloroform anaesthesia. Determination of the concentrations of total proteins, cholesterol, LDL, TAG and HDL were by standard methods. The results showed that the extract caused a dose dependent significant (p < 0.05) increase in the body weight of the test animals when compared with the control group A, but the weight of the liver and kidney did not increase or decrease significantly (p > 0.05). Both extracts contain phenols, flavonoids and vitamin C. However, the concentration of these phytochemicals were more in the aqueous extract, also the aqueous extract exhibited radical scavenging activity than the n-Hexane extract. The treatment of the rats with the aqueous extract produced a dose dependent significant (p < 0.05) increase in the concentration of total proteins and HDL. The treatment significantly (p < 0.05) and in a dose dependent manner decreased the concentration of cholesterol, TAG and LDL. The phenolic content of the extract could be responsible for the radical scavenging activity; extract has lipid lowering properties, which may be enhanced in the management of lipid related disorders.
Adedayo BC, Oboh G &Akindahunsi AA 2010.Changes in the total phenol content and anti-oxidant properties of pepper fruit (Dennettiatripetala) with ripening.Afr. J. Food Sci., 4(6): 403 – 409. Block G, Jensun CD, Norkus EP, Dalvi TB, Wang LG, McmanusJF&Hudes ML 2007. Usage patterns, health and nutritional status of long term multiple details, supplement users: a cross sectional study. Nutritional Journal, 6: 30-40. Copper R 2009. Hyperaldosteronism. Medline plus available at http://www.nlm.nil.gov/medlineplus/ency/article/000330.htm Daniel EI& Clement ON 2008.Effect of ethanolic extract of Dennettiatripetala fruit on haematological parameters in albino Wistar rats.Nig. J. Physiol. Sci., 23(1-2): 13-17. Despress JP 2009. The atherogenic triad of metabolic risk factors: importance of waist and fasting triglyceride as screening tool. Amer. Diabettes Assoc. J., 32(10):12-7. Groove TH 1989. Determination of HDL by precipitation with phosphotunstate magnesium.Clinical chemistry, 41: 91-103. Head I& Kathleen C 2011. Natural therapies for Ocular disorder, part two: cataract and glaucoma. Alternative Review, 6(2): 141-166. Hu S, Nlederau C, KlonowskiSH, Halank W &Lutlen B 2005.Oxidative stress in acute pancreatic hepato-gastroenterology.Journal of physiology, 46: 2736-2750. Idoko JE & Adebayo RA 2011. Effect of Dennettiatripetala powder and reduced rates of pirimiphos-methyl singly and combined in management of callosobruchusmaculatus (F) (Coleoptera: Bruchidae). Intern. J. Bio., 3(2): 174-178 Laguerre M 2007. Lipid peroxidation.Progress in Lipid Research, 36: 244-282. McGregor GP &Biesalski K 2006.Rationale and impact of vitamin C in clinical nutrition.Current Opinion in Clinical Nutrition & Metabolic Care, 9(6): 697-703. Meda A, Lamien CE, Romito M, Millogo J &NacoolmaOG 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina fasan Honey as well as their radical scavenging activity. J. Food Chem., 91: 571-577. Okwu DE &MorahFNI 2004. Mineral and nutritive value of Dennettiatripetalafruits. J. Medical & Aromatic Plant Sci., 59: 437-442. Okwu DE, MorahFNI& Anan EM 2005.Isolation and characterization of phenanthrenic alkaloid Uvariopsine from Dennettiatripetala fruits.J. Medical & Aromatic Plant Sci., 27: 496-498. Singleton VL, Orthofer R &Lamuela–RaventosRM 1999.Analysis of total phenols and other oxidation substrates and anti-oxidants by means of Folin-ciocalteau reagent.Methods in Enzymology, 299: 152-178. Tietz NW 1990. Clinical Guide to Laboratory Test. 2nd edn W.B Saunders Company Philadelphia USA, pp. 554-554.
